Study on determination and pharmacokinetics of garlic saponins PIEB in the rat plasma by LC-MS/MS / 药学实践杂志

ABSTRACT

Objective To establish a method for concentration determination of garlic saponins PIEB in the rat plasma and study the pharmacokinetics of PIEB.Methods The separation was performed on a reverse phase Athena C18-WP (50mm×2.1mm, 3μm), with the mobile phase consisted of elution of acetonitrile-water (contain 0.05％formic acid) at a flow rate of 0.3ml/min.After intravenous and gavage administration, the blood samples of rats were collected in different time, and measured by LC-MS/MS.The pharmacokinetics parameters were calculated by Inna Phase Kinetica2000TMpharmacokinetics software.Results There was a good linearity of the calibration curves over the concentration range of 10-2 430ng/ml (r=0.999 1), and the investigation of methodology all accorded with the demands.After intravenous administration of 50mg/kg PIEB in rats, the maximum concentration of PIEB was achieved immediately.The t1/2of PIEB was about 322minutes and the MRT was 270minutes.After oral administration of low, medium and high concentrations of garlic saponin, the AUC and cmaxincreased with the increasing of dosage, which were consistent with the features of linear pharmacokinetics.Conclusion The established method was simple, accurate and sensitive, which could be suitable for the contents determination of garlic saponins PIEB in the rat plasma.