Determination of fluorine and aluminum concentrations in serum, bone, teeth, brain, kidney and liver tissues of rats with combined fluorine-aluminum toxicosis caused by drinking brick tea / 中华地方病学杂志

ABSTRACT

Objective To observe the effect of fluorine (F) and aluminum (Al) on serum,bone,teeth,brain,kidney and liver tissues,contents of F and Al in different tissues of rats with combined F-Al toxicosis caused by drinking brick tea were determined.Methods Forty SPF grade Wistar rats of 4 weeks,including 24 females and 16 males,weighing between 40 and 50 g,were divided into control group,F group,Al group and brick tea group by random number table according to their weight,ten rats (6 females,4 males) in each group.The control group received daily drinking water.The F group drank a solution with an F content of 100 mg/L,and the Al group drank a solution with an Al content of 159 mg/L.According to the drinking habits and consumption of the herdsmen,the tea concentration and tea consumption of the rats were calculated for the brick tea group,and a tea soup was made according to the ratio,with the F content of (100 ± 2) mg/L and the Al content of (159.0 ± 1.5) mg/L.One year later,the rats were killed to determine the contents of F and Al in serum,bone,teeth,brain,kidney and liver.Results The contents of F in bone,teeth and liver of the brick tea group [(275.81 + 55.89),(283.32 ± 70.67),(15.52 ±7.57) μg/g] were significantly higher than those of the control group [(0.04 ± 0.01),(128.52 ± 12.81),(5.89 ±0.33) μg/g,P ＜ 0.01].The Al contents of teeth and liver in the brick tea group [(6.14 ± 1.69),(8.97 ± 0.85) μg/g]were significantly higher than those of the control group [(0.72 + 0.54),(5.47 + 0.83) μg/g,P ＜ 0.01].Conclusions F-Al combined toxicosis caused by drinking brick tea can cause the increases of F contents in bone,teeth and liver,and the increases of Al contents in teeth and liver.The main tissues damaged by F-Al combined toxicosis caused by drinking brick tea in rats are bone,teeth and liver.