Differential expression of 14-3-3 gene in different stages of Taenia solium / 中华地方病学杂志

ABSTRACT

Objective To investigate the expression difference of 14-3-3 gene in different stages of Taenia solium (Ts),and to provide basic information for exploring the regulation mechanism of Ts14-3-3 gene in growth and development of Ts.Methods Adult worms were collected from patients with taeniasis solium in the taeniasis epidemic area of Yajiang County,Ganzi Tibetan Autonomous Prefecture,Sichuan Province.After determining the species through morphological observation under the light microscope and transcribed spacer 1 (ITS1) method,it was identified as Ts,then piglets were infected to obtain the Cysticercus cellulosae of Ts (larvae).Reverse transcription PCR was used to detect the expression of Ts14-3-3 gene in the adult stage and larval stage of Ts,and the relative expression levels of each were detected by real-time fluorescent quantitative PCR.Results Under light microscope,it could be seen that the collected adult worm scolex consisted of 4 suckers,rostellum and hooks,which was consistent with the typical characteristics of the Ts scolex;the worm sample could amplify the target fragment which was consistent with the length of the ITS1 gene by reverse transcription PCR,and was determined to be Ts.The Ts14-3-3 gene family members Ts14-3-3.1,Ts14-3-3.2,Ts14-3-3.3,Ts14-3-3.4,Ts14-3-3.5 and Ts14-3-3.6 were expressed in the Ts adult stage and larval stage.Compared with the larval stage,the expression levels of Ts14-3-3.1 (0.47 ± 0.09 vs 1.01 ± 0.23),Ts14-3-3.2 (0.31 ± 0.09 vs 1.05 ± 0.14),Ts14-3-3.3 (0.64 ± 0.23 vs 1.26 ± 0.23) and Ts14-3-3.4 (0.30 ± 0.09 vs 0.79 ± 0.23) were significantly decreased in the adult stage (t =-3.816,-7.093,-3.377,-3.481,P ＜ 0.01 or ＜ 0.05);the expression levels of Ts14-3-3.5 (3.59 ± 0.09 vs 0.99 ± 0.12) and Ts14-3-3.6 (5.74 ± 2.76 vs 1.03 ± 0.60) were significantly increased (t =30.714,9.718,P ＜ 0.01).Conclusion The expression of Ts14-3-3 gene has stage specificity and the Ts14-3-3 gene may play a special regulatory role in different stages of Ts.