A study on the oxidative stress induced by ambient fine particulate matter (PM 2 .5)and water-soluble fraction on HBE cells / 预防医学

ABSTRACT

Objective Toinvestigatethecytotoxicityandoxidativestressofambientfineparticulatematter(PM2.5)and water-solublefractionofPM2.5onhumanbronchialepithelialcells(HBE).Methods PM2.5sampleswerecollected in the urban area of Hangzhou.Then the water-soluble fraction was extracted from PM2.5.After HBE cells were exposed to PM2.5 and its water-soluble fraction at the doses of 0,100,250,500,1 000,1 500 and 2 000 μg/mL for 24 h, CCK-8 (cell counting kit-8 )assay was conducted to examine the cytotoxicity of the PM2.5 and its water-soluble fraction.The oxidative damage induced by PM2.5 and its water-soluble fraction on HBE cells was then evaluated with lipid peroxidation,the superoxide dismutase (SOD ) activity,and the levels of glutathione peroxidase (GSH -Px ). Results ThePM2.5anditswater-solublefractionreducedtheviabilityofHBEcellsinadose-dependentmanner. When the PM concentrations were 200,400 and 800 μg/mL,the SOD activity of the HBE cells decreased significantly,as compared with the control group (P<0.05 ).Also,the malondialdehyde (MDA)levels of the HBE cells significantly increased at the doses of 200,400 and 800 μg/mL (P<0.05).However,there were no significant differences of GSH-Pxactivityamongthegroups.Conclusion ThePM2.5anditswater-solublefractioncouldinducecytotoxicand oxidative damage effects on the HBE cells.