An investigation on the infection status and genotype of Hantavirus carried by rodents in Yuyao City of Zhejiang Province / 预防医学

ABSTRACT

Objective To learn the infection status and genotype of Hantavirus carried by rodents in Yuyao City.Methods From May,2014 to May,2015,a total of 248 rodents were captured from four kinds of characteristic topographies (plains, mountains,coastal and urban areas)with clip night method in Yuyao.The real time fluorescence quantitative PCR method was used to detect the Hantavirus from lung specimens of rodents.The RT-PCR method was used to sequence and analyze the M fragment gene.Results Hantavirus nucleic acid was detected positive in 12 samples from a total of 248 specimens, and the positive rate was 4.84%,and they were all detected in Rattus norvegicus and were all Seoul virus (SEOV).Eight samples were sequenced and analyzed,and phylogenetic tree was constructed.The result showed SEOV S3 subtype with high homology.Conclusion Hantavirus carried by rodents in Yuyao City is mainly SEOV S3 subtype,and the distribution of gene subtype is single.Rattus norvegicus is the main host of this virus.