Association of Nitric Oxide Levels and Endothelial Nitric Oxide Synthase G894T Polymorphism with Coronary Artery Disease in the Iranian Population
Vascular Specialist International
;
: 105-112, 2016.
Article
in English
| WPRIM
| ID: wpr-79756
ABSTRACT
PURPOSE:
The endothelial nitric oxide synthase (eNOS) G894T polymorphism has been reported to cause endothelial dysfunction and may have a role in the development of coronary artery disease (CAD). The aim of the present study was to investigate the association of eNOS G894T genetic polymorphism and plasma levels of nitric oxide (NO) with CAD risk in an Iranian population. MATERIALS ANDMETHODS:
We studied 200 patients with angiographically documented CAD and 100 matched controls. Analysis of G894T genetic polymorphism of eNOS was performed by polymerase chain reaction-restriction fragment length polymorphism method. Plasma levels of NO were determined using Griess method. Biochemical analysis was conducted by routine colorimetric methods.RESULTS:
Plasma levels of NO were significantly lower in CAD patients than control subjects (41.60±12.70 vs. 55.48±16.57, P=0.001). Also, the mean plasma levels of NO were significantly lower in T allele carriers of eNOS G894T polymorphism than G allele carriers (P0.05).CONCLUSION:
Reduced plasma level of NO is associated with increased risk of CAD in our population. Moreover, eNOS G894T polymorphism is a significant risk factor for CAD development via reducing the plasma levels of NO. However, eNOS G894T polymorphism is not a contributing factor for the severity of CAD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasma
/
Polymorphism, Genetic
/
Coronary Artery Disease
/
Risk Factors
/
Coronary Vessels
/
Alleles
/
Nitric Oxide Synthase Type III
/
Gene Frequency
/
Genotype
/
Methods
Type of study:
Etiology study
/
Risk factors
Limits:
Humans
Language:
English
Journal:
Vascular Specialist International
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS