Platelet-Rich Fibrin Lysate Can Ameliorate Dysfunction of Chronically UVA-Irradiated Human Dermal Fibroblasts
Yonsei Medical Journal
;
: 1282-1285, 2016.
Article
in English
| WPRIM
| ID: wpr-79762
ABSTRACT
To determine whether platelet-rich fibrin lysate (PRF-L) could restore the function of chronically ultraviolet-A (UVA)-irradiated human dermal fibroblasts (HDFs), we isolated and sub-cultured HDFs from six different human foreskins. HDFs were divided into two groups those that received chronic UVA irradiation (total dosages of 10 J cm-2) and those that were not irradiated. We compared the proliferation rates, collagen deposition, and migration rates between the groups and between chronically UVA-irradiated HDFs in control and PRF-L-treated media. Our experiment showed that chronic UVA irradiation significantly decreased (p<0.05) the proliferation rates, migration rates, and collagen deposition of HDFs, compared to controls. Compared to control media, chronically UVA-irradiated HDFs in 50% PRF-L had significantly increased proliferation rates, migration rates, and collagen deposition (p<0.05), and the migration rates and collagen deposition of chronically UVA-irradiated HDFs in 50% PRF-L were equal to those of normal fibroblasts. Based on this experiment, we concluded that PRF-L is a good candidate material for treating UVA-induced photoaging of skin, although the best method for its clinical application remains to be determined.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Skin
/
Time Factors
/
Ultraviolet Rays
/
Blood Platelets
/
Fibrin
/
Cell Movement
/
Cells, Cultured
/
Collagen
/
Cell Proliferation
/
Fibroblasts
Limits:
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
2016
Type:
Article
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