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Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells
Journal of Veterinary Science ; : 369-378, 2004.
Article in English | WPRIM | ID: wpr-79776
ABSTRACT
Evidences show that eukaryotic mRNAs can perform protein translation through internal ribosome entry sites (IRES). 5'-Untranslated region of the mRNA encoding apoptotic protease-activating factor 1 (Apaf-1) contains IRES, and, thus, can be translated in a cap-independent manner. Effects of changes in protein translation pattern through rapamycin pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-butanone(NNK, tobacco-specific lung carcinogen)-induced apoptosis in human bronchial epithelial cells were examined by caspase assay, FACS analysis, Western blotting, and transient transfection. Results showed that NNK induced apoptosis in concentration- and time-dependent manners. NNK-induced apoptosis occurred initially through cap-independent protein translation, which during later stage was replaced by cap-dependent protein translation. Our data may be pplicable as the mechanical basis of lung cancer treatment.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Time Factors / Protein Biosynthesis / Bronchi / Carcinogens / Proteins / Carrier Proteins / Blotting, Western / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Sirolimus Limits: Humans Language: English Journal: Journal of Veterinary Science Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Time Factors / Protein Biosynthesis / Bronchi / Carcinogens / Proteins / Carrier Proteins / Blotting, Western / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Sirolimus Limits: Humans Language: English Journal: Journal of Veterinary Science Year: 2004 Type: Article