Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells
Journal of Veterinary Science
;
: 369-378, 2004.
Article
in English
| WPRIM
| ID: wpr-79776
ABSTRACT
Evidences show that eukaryotic mRNAs can perform protein translation through internal ribosome entry sites (IRES). 5'-Untranslated region of the mRNA encoding apoptotic protease-activating factor 1 (Apaf-1) contains IRES, and, thus, can be translated in a cap-independent manner. Effects of changes in protein translation pattern through rapamycin pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-butanone(NNK, tobacco-specific lung carcinogen)-induced apoptosis in human bronchial epithelial cells were examined by caspase assay, FACS analysis, Western blotting, and transient transfection. Results showed that NNK induced apoptosis in concentration- and time-dependent manners. NNK-induced apoptosis occurred initially through cap-independent protein translation, which during later stage was replaced by cap-dependent protein translation. Our data may be pplicable as the mechanical basis of lung cancer treatment.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Time Factors
/
Protein Biosynthesis
/
Bronchi
/
Carcinogens
/
Proteins
/
Carrier Proteins
/
Blotting, Western
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Sirolimus
Limits:
Humans
Language:
English
Journal:
Journal of Veterinary Science
Year:
2004
Type:
Article
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