Effect of Jiawei-Danshen Yin on anti-myocardial fibrosis and Notch signaling pathway in rats with myocardial fibrosis / 国际中医中药杂志

ABSTRACT

Objective@#To study the effect of Jiawei-Danshen Yin on anti-myocardial fibrosis and Notch signaling pathway in rats with myocardial fibrosis.@*Methods@#The rats were randomly divided into normal group (10 rats), model group (5 rats), latopril group (5 rats), low dose group (5 rats) and high dose group (5 rats). In addition to the normal group, the other groups of rats prepared myocardial fibrosis model. One hour after model establishment, the rats in high and low dosage groups were given 1.5 mg/ml and 3.0 mg/ml of modified salvia miltiorrhiza drink respectively, the rats in captopril group were given 1 mg/ml of captopril, the rats in model group and normal group were given saline of equal volume for 1 day/time for one week. The collagen volume fraction (CVF) was measured by pathological observation. The left ventricular mass index (LVMI), heart mass index (HWI), heart rate (HR), left ventricular systolic pre-existing (LVSP) left ventricular end diastolic pressure (LVEDP) and left ventricular systolic pressure were measured. Western blot was used to detect the expression of Notch1, NICD, Notch3 and α-SMA.@*Results@#Compared with the model group, the levels of LVMI (2.26 ± 0.01 mg/g, 1.48 ± 0.03 mg/g vs. 2.86 ± 0.06 mg/g), CVF (3.44% ± 1.45%, 2.62% ± 1.43% vs. 7.82% ± 1.95%), HWI (4.05 ± 0.02 mg/g, 3.11 ± 0.02 mg/g vs. 4.58 ± 1.05 mg/g) in the low and high dose groups of Jiawei-Danshen Yin significantly decreased respectively(P<0.05). The levels of LVEDP (10.16 ± 2.35 kPa, 6.25 ± 1.23 kPa vs. 13.15 ± 3.06 kPa) significantly decreased, HR (375.22 ± 20.36 time/min, 450.32 ± 22.13 time/min vs. 329.36 ± 22.01 time/min), LVSP (90.12 ± 6.32 kPa, 110.45 ± 10.71 kPa vs. 80.54 ± 10.35 kPa) significantly increased (P<0.05) respectively. The expression of Notch1 (0.83 ± 0.05, 1.11 ± 0.04 vs. 0.51 ± 0.06), NICD (0.72 ± 0.01, 1.06 ± 0.03 vs. 0.35 ± 0.05), Notch3 (0.62 ± 0.03, 1.20 ± 0.06 vs. 0.39 ± 0.05) in myocardial tissue significantly increased, and the expression of α-SMA (1.12 ± 0.08, 0.92 ± 0.05 vs. 1.43 ± 0.07) significantly decreased (P<0.05).@*Conclusions@#The Jiawei-Danshen Yin has a significant effect on myocardial fibrosis model rats. It can improve the anti-myocardial fibrosis ability by activating Notch signaling pathway.