Effect of Huangqi Guizhi Wuwu Tang on Angiogenesis in Osteoarthritis / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
;
(24): 87-93, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-798357
ABSTRACT
Objective:
To study the effect of Huangqi Guizhi Wuwu Tang on angiogenesis of osteoarthritis with Yang deficiency and cold coagulation.Method:
Totally 60 female SD rats were randomly divided into control group, model group, celecoxib group (20.82 mg·kg-1) and low, medium, high-dose Huangqi Guizhi Wuwu Tang groups (3.24, 6.48, 12.96 g·kg-1). All groups, except for control group, were involved in duplicating the osteoarthritis(OA) model through frozen and knee fixation, as well as 42-day cold environmental stimulation. After modeling, all drug-group rats were respectively administrated with corresponding drugs for 28 days, once a day. Meanwhile, control group and model group were given equivalent distilled water by gavage. 24 hours after the last gavage, vascular endothelial growth factor (VEGF),prostaglandin E2(PGE2) and transforming growth factor-β1(TGF-β1) in serum were detected with enzyme linked immunosorbent assay(ELISA) method, VEGF expressions in cartilage and synovial with immunohistochemical method, and interleukin-17(IL-17) and VEGF levels in synovial with Real-time fluorescence quantitative polymerase chain reaction(Real-time PCR).Result:
Compared with normal group, the expression of VEGF in serum, cartilage and synovial were significantly increased (P2 and TGF-β1 expressions, IL-17 level were increased significantly (P2, TGF-β1 and IL-17 level were decreased significantly (PPPConclusion:
Huangqi Guizhi Wuwu Tang has an effect in suppressing angiogenesis of knee and alleviate cartilage lesion by regulating VEGF and its upstream cytokines PGE2 and TGF-β1.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Chinese Journal of Experimental Traditional Medical Formulae
Year:
2019
Type:
Article
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