Analysis and Comparison of Volatile Components in Trifolium pratense and Trifolium repens Seed by GC-MS / 中国实验方剂学杂志

ABSTRACT

Objective: To analyze and compare the difference of volatile components between Trifolium pratense and T. repens seeds.Method: The volatile components were extracted by the headspace solid phase microextraction (HS-SPME) method and analyzed by gas chromatography-mass spectrometry (GC-MS),and the relative contents of the components were calculated by the area normalization method.Result: Fourty six compounds were identified in the seeds of T. pratense,with a total peak area of 3.48×109 for the volatile components. The compounds with high content in this species were 1-octen-3-ol (30.24%),1-hexanol (11.94%),heptane,2,2,4,6,6-pentamethyl (11.00%),nonanal (7.74%),octanal (6.79%) and hexanoic acid (6.04%). 44 compounds were identified from the seeds of T. repens,with a total peak area of 6.37×109 for the volatile components. The compounds with high content in this species were 1-octen-3-ol (42.98%),heptadecane (14.47%),3-octanol (9.42%),n-caproic acid vinyl ester (8.82%) and pyrimidine-4,6-diol,5-methyl (6.59%). 20 components were commonly shared in the seeds of T. pratense and T. repens,but the content of each component was quite different. 1-octen-3-ol had the highest content in the common components. T. pratense seeds had 26 unique components,accounting for 54.85%of volatile components. T. repens seeds had 24 unique components,accounting for 46.79%of volatile components.Conclusion: The seeds of both T. pratense and T. repens have rich volatile components,but there are great difference in the variety,peak area and relative content of the volatile components between these two varieties