Effect of Buzhong Yiqi Tang on Cisplatin Resistance A549/DDP Cells in Nude Mice with Lung Adenocarcinoma / 中国实验方剂学杂志

ABSTRACT

Objective: To investigate the effect of Buzhong Yiqi Tang on cisplatin resistance tumor cells A549/DDP in nude mice with lung adenocarcinoma, and determine the optimal concentration. Method: The 36 BALB/C nude mice were randomly divided into no-load group, model group, cisplatin group, low-dose Buzhong Yiqi Tang group (1.46 g · kg-1), medium-dose Buzhong Yiqi Tang group (2.92 g · kg-1) and high-dose Buzhong Yiqi Tang group (5.84 g · kg-1). The no-load group was inoculated with A549/DDP cells transfected by uninfected lentiviral transforming growth factor-β1(TGF-β1), and the other groups were inoculated with TGF-β1 over-expression A549/DDP cells. After the intervention, the nude mice were put to death. The tumors of mice were weighed, and the inhibition rate was calculated, liver, spleen and lung metastases of tumors were counted under microscope, and pathological observation was made for lung histomorphological changes. Immunohistochemistry, Western blot and Real-time polymerase chain reaction(Real-time PCR) were used to detect the expressions of lung resistance-related protein (LRP), multidrug resistance-related protein (MRP), P-glycoprotein (P-gp) in transplantation tumor. Result: Compared with the model group, the weight of tumors and the number of pulmonary metastatic nodules decreased in the Buzhong Yiqi Tang intervention group. With the increase of the dosage of Buzhong Yiqi Tang, the weight of tumors gradually decreased, while the inhibition rate increased. The effect of the high-dose group was the most significant (PPPConclusion: Buzhong Yiqi Tang can inhibit the growth and metastasis of transplanted tumors, alleviate lung injury and improve the drug resistance of A549/DDP to cisplatin, with a certain dose-effect relationship. High-dose concentration (5.84 g ·kg-1) was the best concentration in nude mice.