Isolation and Identification of Chemical Constituents from Cyclosorus parasiticus / 中国实验方剂学杂志

ABSTRACT

Objective:To study the chemical constituents from the ethyl acetate extract of Cyclosorus parasiticus. Method:The whole plant of C. parasiticus were repeatedly percolated with MeOH. The MeOH residue was dispersed in H2O and successively extracted with petroleum ether,ethyl acetate and butyl alcohol. The chemical constituents from the ethyl acetate fraction were isolated by silica gel,Sephadex LH-20 and ODS columns,and semi-prep high performance liquid chromatography,and their chemical structures were determined on the basis of IR,MS,NMR analysis (1H and 13 C-NMR) and spectroscopic data from literatures. Result:Sixteen compounds were isolated from the ethyl acetate fraction, and their chemical structures were identified as physcion (1),ent-kaur-16-ene-2β,15α-diol (2),ent-3β-hydroxy-kaur-16-ene (3),5,7,8,4'-tetramethoxyflavone (4),oleanolic acid (5),5-hydroxy-7,4'-dimethoxyflavone (6),1,7-dihydroxyxanthone (7),interruptin B (8),quercetin (9),apigenin (10),kaempferol (11),afzelin (12),apigenin-7-O-β-D-glucopyranosid (13),kaempferol-7-O-β-D-glucopyranoside (14),β-daucosterol (15) and β-sitosterol (16). Conclusion:Compounds 1-7 and 9-15 were isolated from the C. parasiticus for the first time. These results provide a foundation for studying the pharmacodynamics material basis and quality control of C. parasiticus.