Discussion About Application of Thin Layer Chromatography in Current Quality Standard Control / 中国实验方剂学杂志

ABSTRACT

The application of thin layer chromatography(TLC) in Chinese Pharmacopoeia experienced from scratch to less to more,but the proportion of thin layer chromatographic scanning used in recent editions of Chinese Pharmacopoeia has decreased.With the further improvement of the quality standard system of traditional Chinese medicine(TCM),the shortcomings of TLC identification methods have been gradually revealed,such as the low penetration rate of instrument,poor repeatability and stability of the results,lower identification speed than high performance liquid chromatography(HPLC) and so on.Thus it gradually became untimely.In the process of formulating quality standard control of TCM,researchers should not be conformist,and TLC identification should not be a necessary choice for the qualitative identification.And HPLC has the possibility to completely replace TLC,but TLC can be used as a supplement to HPLC.In order to fully lower testing cost,shorten testing cycle,improve the efficiency of identification,we suggested quality standard system of TCM should be sharply reduced TLC identification method and increased identification of HPLC characteristic spectrum;and try to do "one condition,one map".Unless absolutely necessary,national quality standards(such as Chinese Pharmacopoeia) should use TLC identification as a recommended method rather than a mandatory standard.