Research on Profile Differences of Chemical Components in Magnoliae Officinalis Cortex from Different Varieties and Habitats by LC-TOF-MS / 中国实验方剂学杂志

ABSTRACT

Objective:To study on the differences of profile spectra among chemical components in Magnoliae Officinalis Cortex from different varieties and habitats,and to screen and identify the characteristic components affecting the quality difference of this herb. Method:The chromatogram data sets of Magnoliae Officinalis Cortex from different varieties and habitats were obtained by liquid chromatography-time-of-flight-mass spectrometry(LC-TOF-MS).Principal component analysis,partial least squares-discriminant analysis and cluster analysis were used to compare the differences in chemical profiles among Magnoliae Officinalis Cortex from different varieties and habitats,and adopted to screen out the characteristic chemical constituents that resulted in these differences and to perform mass spectrometry analysis and comparison. Result:Eleven characteristic peaks were identified by LC-TOF-MS chromatographic data and reported in the literature.The use of chemical profile could distinguish different habitats of Magnoliae Officinalis Cortex,but could not completely distinguish different varieties of this herb. Conclusion:LC-TOF-MS can easily and quickly study on the profile differences of chemical substances in Magnoliae Officinalis Cortex from different varieties and different habitats,the results of this study can provide a theoretical basis for the quality evaluation and pharmacodynamic material basis of this herb.