Effect of Modified Shaoyao Gancao Tang on MPTP-induced Parkinson's Disease Model Mice / 中国实验方剂学杂志

ABSTRACT

Objective:To study the effect of modified Shaoyao Gancao Tang on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease model mice. Method:The sixty C57/BL6 mice were randomly divided into normal group, model group, Madopar group (50 mg·kg-1) and low, medium and high doses modified Shaoyao Gancao Tang (1,2,4 g·kg-1). The modeling method was to intraperitoneally inject C57/BL6 mice with MPTP(40 mg·kg-1) once a day for 7 days. Except normal group and model group were given normal saline daily,drug-administered group was intragastrical administered once a day, and the third day after the drug was administered according to the above method (except normal group). The behavior of each group of mice (climbing test, swimming test, and tail suspension test) was examined up to 15 days after administration. Subsequently, the levels of Cystatin-C (Cys-C) and neuron-specific enolase (NSE) in the serum of each group were detected by enzyme-linked immunosorbent assay (ELISA). The levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in striatum of each group were detected by spectrophotometry. The protein expression of α-synuclein (α-syn), tyrosine hydroxylase (TH),B lymphoma-2 gene (Bcl-2) and Bcl-2 related X protein (Bax) in the striatum of each group was detected by Western blot. Result:Compared with normal group, the swimming experiment scores and suspension experiments scores in the behavioral experiments of model group mice were significantly lower (PPPPPPPα-syn and Bax expression in striatum were significantly increased (PPPPPPPPα-syn and Bax expression in striatum were significantly decreased (PPPPPPPPα-syn expression and Bax expression in the striatum were significantly decreased (PConclusion:The neuroprotective effect of modified Shaoyao Gancao Tang on MPTP-induced Parkinson's disease model mice may be achieved by inhibiting oxidative damage and apoptosis.