Effect of Shenling Baizhu San on Murine Model of IBD Induced by DSS in Mice by Regulating Autophagy / 中国实验方剂学杂志

ABSTRACT

Objective: To investigate the mechanism of Shenling Baizhu San (SLBZS) in treating dextra sulfate sodium (DSS)-induced inflammatory bowel disease (IBD) mice and its relationship with autophagy. Method: SPF BALB/c mice were randomly divided into control group,model group,mesalazine group,low,medium and high-dose SLBZS groups,and autophagy inducer rapamycin group.The IBD mice were fed with 5% DSS in their drinking water for 7 days,and the control mice received only water.SLBZS groups were given SLBZS at doses of 3,6,12 g·kg-1·d-1, positive group was given mesalazine sustained release granules at the dose of 2 g·kg-1·d-1, rapamycin group was given rapamycin at the dose of 4 mg·kg-1·d-1, and control mice was given the same volume of normal saline by gavage.The mice weight,stool occult blood in stool,score of disease activity (DAI),pathological examination of intestinal mucosal lesions integral were observed after 7 days. interleukin(IL)-8 and IL-10 in serum were detected by enzyme\|linked immuno sorbent assay(ELISA), vascular tissue samples were prepared for the detection of tumor neorosis factor-(TNF-α) and IL-1β, and transmission electron microscope and Western blot were used to detect the formation of autophagosomes and the level of autophagy. Result: The body mass decrease, the colon length, disease activity scoring, and histological scoring of SLBZS group were better than those of DSS group. Compared with control group, the level of IL-10 decreased, while the level of IL-8 increased obviously (Pα expressions significantly up-regulated(PPPα expressions(PConclusion: Shenling Baizhu San can significantly inhibit the IBD by regulating autophagy and suppressing inflammation.