Effect of Sanshencao Essential Oil on Rat Model of Thromboangiitis Obliterans / 中国实验方剂学杂志

ABSTRACT

Objective: To observe the effect of Sanshencao essential oil on the model of thromboangiitis obliterans in rats. Method: Male Wistar rats were randomly divided into five groups according to body weightsham operation group, model group, Tongsaimai group (1.05 g·kg-1), large and small-dose Sanshencao essential oil groups (0.125 mL·cm-2, twice a day, 0.125 mL·cm-2, once a day), with 10 in each group. The model of occlusive vasculitis was established by injecting sodium laurate solution (10 g·L-1) into the left lower limb artery of rats. Sham operation group was injected with the same amount of normal saline at the same position. Large and small doses of Sanshencao essential oil were applied directly to the affected area, and the remaining groups were given corresponding drugs or pure water for 10 days. One hour after the last administration, the pathological changes were graded; the levels of thromboxane B2(TXB2), interleukin-1 (IL-1), 6-oxo-prostaglandinF1α (6-K-PGF1α), endothelin (ET) were measured by radioimmunoassay. Hematoxylin-eosin(HE) staining was used to observe the pathological changes of the femoral arteries in the left lower limbs. Result: Compared with sham operation group, the degree, extent, inflammation and thrombosis of the affected limbs in model group increased significantly (PPPP2, ET and IL-1 in serum (PP1α (PPConclusion: Sanshencao essential oil has a good therapeutic effect on thrombosis angiitis obliterans model in rats, which related to its anti-oxidation and alleviating inflammation of vascular wall.