Chemical Compositions and Antioxidant Activity of Volatile Oils from Doellingeria scaber / 中国实验方剂学杂志

ABSTRACT

Objective: To identify the chemical compositions and conduct relative content analysis of the essential oil from wild Doellingeria scaber in high altitude area of Dabie mountains, and investigate its antioxidant capacity. Method: The volatile oil of D. scaber was extracted by simultaneous distillation extraction (SDE) method. Its chemical compositions were analyzed by gas chromatography-mass spectometry (GC-MS), and the relative percentage content of each composition was determined by peak area normalization method. The antioxidant activities of the oil were evaluated by reducing ability method, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) method and β-carotene bleaching assay. Result: A total of 50 components representing 86.91% in this plant were identified. The main chemical compositions were (E)-β-farnesene(20.21%), germacrene D(9.94%), hexadecanoic acid(8.66%), β-terpinene(7.82%), caryophyllene(6.9%) and p-cymen-8-ol(4.48%). The main components of volatile oils include alkenes (56.57%), alcohols (12.24%), fatty acids (11.24%), epoxides(2.93%), esters(1.82%), aldehydes and ketones (1.60%), and aromatics (0.51%). The reduction ability of volatile oil to iron ions increases with the increase of concentration, but the reduction ability is far weaker than that of vitamin C. The 50% inhibiting concentration (IC50) of volatile oil on DPPH free radicals and in β-carotene/linoleic acid lipid system was 0.72 g·L-1 and 0.10 g·L-1, respectively, indicating that the volatile oil had a certain scavenging capacity on DPPH free radicals and a good inhibition effect on lipid peroxidation. Conclusion: GC-MS was used to identify the chemical compositions of the volatile oil from D. scaber, and antioxidant test in vitro showed that D. scaber had certain antioxidant capacity, providing experimental basis for its development and utilization.