Expression pattern of the class I homeobox genes in ovarian carcinoma / 부인종양
Journal of Gynecologic Oncology
;
: 29-37, 2010.
Article
in English
| WPRIM
| ID: wpr-8032
ABSTRACT
OBJECTIVE:
Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis.METHODS:
The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries.RESULTS:
Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries.CONCLUSION:
We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Ovary
/
RNA, Messenger
/
Carbonates
/
Cell Line
/
Genes, Homeobox
/
Blotting, Western
/
Aluminum Hydroxide
Language:
English
Journal:
Journal of Gynecologic Oncology
Year:
2010
Type:
Article
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