The inhibition of inflammatory molecule expression on 3T3-L1 adipocytes by berberine is not mediated by leptin signaling
Nutrition Research and Practice
; : 84-88, 2009.
Article
in En
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| ID: wpr-80366
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ABSTRACT
In our previous study, we have shown that berberine has both anti-adipogenic and anti-inflammatory effects on 3T3-L1 adipocytes, and the anti-adipogenic effect is due to the down-regulation of adipogenic enzymes and transcription factors. Here we focused more on anti-inflammatory effect of berberine using real time RT-PCR and found it changes expressions of adipokines. We hypothesized that anti-adipogenicity of berberine mediates anti-inflammtory effect and explored leptin as a candidate mediator of this signaling. We studied this hypothesis by western blot analysis, but our results showed that berberine has no effect on the phosphorylations of STAT-3 and ERK which have important roles on leptin signaling. These results led us to conclude that the anti-inflammatory effect of berberine is not mediated by the inhibition of leptin signal transduction. Moreover, we have found that berberine down-regulates NF-kappaB signaling, one of the inflammation-related signaling pathway, through western blot analysis. Taken together, the anti-inflammatory effect of berberine is not mediated by leptin, and berberine induces anti-inflammatory effect independent of leptin signaling.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Phosphorylation
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Transcription Factors
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Berberine
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Signal Transduction
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Down-Regulation
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Blotting, Western
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NF-kappa B
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Adipocytes
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Leptin
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Adipokines
Language:
En
Journal:
Nutrition Research and Practice
Year:
2009
Type:
Article