Mechanism of lncRNA CTD-3252C9.4 on invasion and migration of pancreatic cancer Panc-1 cells in vitro / 中国药科大学学报

ABSTRACT

@#The aim of this study is to investigate the inhibitory effect of long non-coding RNA(lncRNA)CTD-3252C9. 4 on migration and invasion of human pancreatic cancer cell Panc-1 in vitro and its mechanism. Panc-1 cells were stimulated by epidermal growth factor(EGF)in three-dimensional semi-solid system of cultured pancreatic cancer spheres. RT-qPCR was used to detected the transfection efficiency of lncRNA CTD-3252C9. 4. The effects of lncRNA CTD-3252C9. 4 and bone morphogenetix protein 7(BMP7)on the invasion and migration of Panc-1 cells were detected by scratch healing method and Transwell chamber method. The changes of target gene BMP7 and epithelial-mesenchymal transition(EMT)related proteins were verified by Western blot. EGF could significantly inhibit the expression of lncRNA CTD-3252C9. 4 in Panc-1 cells. The lncRNA can affect cells invasion and migration by inhibiting the transcription of the oncogene BMP7, then inhibit the process of EMT of tumors.