COMP-Angiopoietin-1 Promotes Cavernous Angiogenesis in a Type 2 Diabetic Rat Model
Journal of Korean Medical Science
;
: 725-730, 2013.
Article
in English
| WPRIM
| ID: wpr-80576
ABSTRACT
Cartilage oligomeric matrix protein-angiopoietin-1 (COMP-Ang1) is an angiogenic factor for vascular angiogenesis. The aim was to investigate the effect of an intracavernosal injection of COMP-Ang1 on cavernosal angiogenesis in a diabetic rat model. Male Otsuka Long-Evans Tokushima Fatty (OLETF) rats made up the experimental group (1 yr old) and Long-Evans Tokushima Otsuka (LETO) rats made up the control group. The experimental group was divided into vehicle only, 10 microg COMP-Ang1, and 20 microg COMP-Ang1. COMP-Ang1 was injected into the corpus cavernosum of the penis. After 4 weeks, the penile tissues of the rats were obtained for immunohistochemistry and Western blot analysis. The immunoreactivity of PECAM-1 and VEGF was increased in the COMP-Ang1 group compared with the vehicle only group. Moreover, the expression of PECAM-1 and VEGF was notably augmented in the 20 microg Comp Ang-1 group. In the immunoblotting study, the expression of PECAM-1 and VEGF protein was significantly less in the OLEFT rats than in the control LETO rats. However, this expression was restored to control level after intracavernosal injection of COMP-Ang1. These results show that an intracavernosal injection of COMP-Ang1 enhances cavernous angiogenesis by structurally reinforcing the cavernosal endothelium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penis
/
Blood Glucose
/
Body Weight
/
Recombinant Fusion Proteins
/
Immunohistochemistry
/
Blotting, Western
/
Neovascularization, Physiologic
/
Platelet Endothelial Cell Adhesion Molecule-1
/
Rats, Long-Evans
/
Angiopoietin-1
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2013
Type:
Article
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