20% Alcohol Toxicity on Rabbit Corneal Epithelial Cells: Electron microscopic study
Journal of the Korean Ophthalmological Society
;
: 751-761, 2001.
Article
in Korean
| WPRIM
| ID: wpr-80581
ABSTRACT
PURPOSE:
To evaluate 20% ethanol toxicity on the rabbit corneal epithelium, ethanol-treated rabbit corneas were examined with electron microscopy.METHODS:
Rabbit corneas(24 eyes) were treated with 20% ethanol for 30 seconds, 1 minute, and 2 minutes by using LASEK(Laser Assisted Subepithelial Keratomileusis) instruments, and washed with sterile water. Zero time, 1, 3, 5 days after ethanol treatment, corneas were excised and examined with scanning and transmission electron microscopy.RESULTS:
Widespread damage or disappearance of microvilli and local breaks of intercellular junction were observed. The changes were more severe in corneas with longer ethanol treament. In corneas with over 1 minute ethanol treatment, slough of superficial corneal epithelium was shown and increased with time. It was difficult to recognize microvilli or distinctive intercellular junction in corneas with 2 minute-treament. These pathologic changes persisted 5 days after ethanol-treatment.CONCLUSIONS:
From these results, 30 seconds to 1 minute-ethanol treatment is recommended in corneal surgery to avoid severe, persisting damage of superficial corneal epithelium.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Water
/
Microscopy, Electron
/
Epithelium, Corneal
/
Cornea
/
Microscopy, Electron, Transmission
/
Ethanol
/
Epithelial Cells
/
Intercellular Junctions
/
Microvilli
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2001
Type:
Article
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