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Detection and clinical significance of differentially expressed microRNAs in chronic hepatitis B patients before being treated with pegylated interferon / 中华实验和临床病毒学杂志
Article in Zh | WPRIM | ID: wpr-806039
Responsible library: WPRO
ABSTRACT
Objective@#To detect differentially expressed microRNAs in chronic hepatitis B (CHB) before being treated with pegylated interferon (PegIFN) and the relationship between their target genes and HBsAg loss.@*Methods@#Pretreatment differentially expressed microRNAs between different response groups were screened using high throughput microarrays and validated by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Bioinformatics analysis was performed to determine their target genes potential mechanistic roles.@*Results@#A total of 417 microRNA were differentially expressed between different response groups, among which 342 were up-regulated and 75 were down-regulated. miR-3960, miR-126-3p, miR-23 a-3p and miR-335-5p were verified to be down-regulated by RT-qPCR result in HBsAg loss group. Bioinformatic analysis result show that the relevant pathways of microRNAs include AMPK signal pathway, NOD-like signal pathway, NF-kappa B signal pathway and mTOR signal pathway.@*Conclusions@#HBsAg loss is probably achieved as the result of genes expression regulated in association with immune response, further enhance the immune response of HBV elimination and acquire HBsAg loss.
Key words
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Experimental and Clinical Virology Year: 2018 Type: Article
Full text: 1 Index: WPRIM Type of study: Diagnostic_studies Language: Zh Journal: Chinese Journal of Experimental and Clinical Virology Year: 2018 Type: Article