Eosinophils Modulate CD4+ T Cell Responses via High Mobility Group Box-1 in the Pathogenesis of Asthma
Allergy, Asthma & Immunology Research
;
: 190-194, 2015.
Article
in English
| WPRIM
| ID: wpr-80637
ABSTRACT
Eosinophils have been reported to modulate T cell responses. Previously, we reported that high-mobility group box 1 protein (HMGB1) played a key role in the pathogenesis of asthma. This study was conducted to test our hypothesis that eosinophils could modulate T cell responses via HMGB1 in the pathogenesis of asthma characterized by eosinophilic airway inflammation. We performed in vitro experiments using eosinophils, dendritic cells (DCs), and CD4+ T cells obtained from a murine model of asthma. The supernatant of the eosinophil culture was found to significantly increase the levels of interleukin (IL)-4 and IL-5 in the supernatant of CD4+ T cells co-cultured with DCs. HMGB1 levels increased in the supernatant of the eosinophil culture stimulated with IL-5. Anti-HMGB1 antibodies significantly attenuated increases of IL-4 and IL-5 levels in the supernatant of CD4+ T cells co-cultured with DCs that were induced by the supernatant of the eosinophil culture. In addition, anti-HMGB1 antibodies significantly attenuated the expressions of activation markers (CD44 and CD69) on CD4+ T cells. Our data suggest that eosinophils modulate CD4+ T cell responses via HMGB1 in the pathogenesis of asthma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Dendritic Cells
/
T-Lymphocytes
/
Interleukins
/
Interleukin-4
/
Interleukin-5
/
HMGB1 Protein
/
Eosinophils
/
Inflammation
/
Antibodies
Type of study:
Etiology study
/
Prognostic study
Language:
English
Journal:
Allergy, Asthma & Immunology Research
Year:
2015
Type:
Article
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