Study on the determination of pyrrole adduct content in hair samples / 中华劳动卫生职业病杂志

ABSTRACT

Objective@#To find out a method to determine the pyrrole adducts in the hairs.@*Methods@#Collected the hair from common people and rats, defatted after completely washed, steeped the hair in different concentration of 2, 5-hexandione to build hair model containing pyrrole adducts; dissolved the hair and determined the concentration of pyrrole adducts.@*Results@#(1) . The combination of 0.72 mol/L of sodium hydrate and 2% tyrisin could dissolve the hair, and the digestion liquid could react with the Ehrlich's reagent showing fuchsia color; (2) . The color could maintain longer after adding more ethanol; (3) . More pyrrole adducts would be produced by the increasing the concentration of 2, 5-dihexandione (P<0.01) ; (4) . Concentration of pyrrole adducts in n-hexane treated hair showed no difference compared with control (P>0.05) .@*Conclusion@#the method could be used to determine the concentration of pyrrole adducts in hair exposed to n-hexane