The effect and mechanism of PinX1 on migration and invasion of melanoma cells / 中华整形外科杂志

ABSTRACT

Objective@#To explore the role of PinXl in the migration and invasion of melanoma cells, and study the mechanism from the perspective of extracellular matrix .@*Methods@#PinX1-siRNA was transfected into melanoma cells A375 and MV3 respectively. Detect the effect of PinXl on proliferation abilities of melanoma cells A375 and MV3 by CCK-8 assay. The migration and invasion abilities of the two cell lines were detected by Transwell migration and invasion assay experiments.The protein expression levels of PinXl, TIMP-1 and MMP-9 were detected by Western Blot analysis.@*Results@#The proliferation, migration and invasion abilities of melanoma cells A375 and MV3 were significantly increased after PinX1 gene knockdown. The expression level of MMP-9 was significantly increased (P<0.01) and the expression level of TIMP-l was reduced significantly(P<0.01).@*Conclusions@#Down-regulation of PinX1 may activate the MMP-9 pathway by down-regulating the TIMP-1 protein, which improve the migration and invasion ability of melanoma cells.