Effect of Growth Hormone on Death of Sertoli Cell / 대한소아내분비학회지
Journal of Korean Society of Pediatric Endocrinology
;
: 184-189, 2002.
Article
in Korean
| WPRIM
| ID: wpr-80688
ABSTRACT
PURPOSE:
Growth hormone(GH) receptors have been detected in Sertoli cells and clinical observations suggested the involvement of GH action(either indirectly via IGF-I or directly) on testicular function. This study is aimed at assessing the effect of GH on cell death during Sertoli cell culture.METHODS:
Sertoli cells(TM4, mouse, male, ATCC, USA) were cultured by using DMEM(Dulbecco' Modified Eagle' Medium, Biowhitteker, USA) and Fetal Bovine Serum(10%, GIBCO BRL, USA) with or without human recombinant growth hormone(Eutropin, LGCI, Korea) of different concentrations(10, 50, 100, 500 microgram/mL of media), in 34degress C, 5% CO2 incubator. After cultivation during 5 days, Sertoli cells were examined under light microscope(x400).RESULTS:
Number of survival Sertoli cell was remarkably increased in the GH treated(50 microgram/mL in media) group(62.6+/-12.4) compare to control(22.4+/-4.8)(P<0.05). Degree of survival Sertoli cell was increased proportionaly until 50 microgram/mL in concentration of GH in media, but decreased in higher than it.CONCLUSION:
GH has the protective effect on death of Sertoli cell and it depends on GH concentration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sertoli Cells
/
Insulin-Like Growth Factor I
/
Growth Hormone
/
Cell Death
/
Cell Culture Techniques
/
Incubators
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Journal of Korean Society of Pediatric Endocrinology
Year:
2002
Type:
Article
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