Affinity characterization of GE11 peptide to brain metastases from epidermal growth factor receptor-overexpressing human breast cancer / 中华核医学与分子影像杂志

ABSTRACT

Objective@#To investigate the feasibility of GE11 peptide targeting to brain metastases from epidermal growth factor receptor (EGFR)-positive breast cancer and thereby explore the potential for the construction of specific targeting molecular probe.@*Methods@#The EGFR expression level of triple negative breast cancer cell line (MDA-MB-231-BR) was detected using Western blot and flow cytometry. GE11 peptide targeting property was tested using immunofluorescence staining and flow cytometry in MDA-MB-231-BR cells and in the subcutaneous tumor model. GK-13 peptide was selected as the control peptide. Two-sample t test was used for data analysis.@*Results@#Western blot and flow cytometry showed that EGFR expression in MDA-MB-231-BR cells was 88.60%, significantly higher than those in other cells. Flow cytometry results indicated that the cell binding rate of MDA-MB-231-BR with GE11 peptide (10 μmol/L) was significantly higher than that with control peptide (38.4% vs 0.6%; t=16.941, P<0.01). Cell immunofluorescence staining results showed GE11 peptides were detected with stronger signal after being incubated with cells, and the combination was concentration- and temperature-dependent. In vivo experiment results showed that the signal of GE11 peptide was stronger in tumor site than that in other organs or tissues.@*Conclusion@#GE11 peptide may specifically target the brain metastases of EGFR-positive breast cancer.