Typing analysis and detection of HPV for condyloma acuminata tissues of perianal / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To learn the prevalence of HPV infection and its genotypes distribution in perianal warts, discuss the association between HPV infection and age and gender, and assess the methodology performance of PCR-reverse dot blot(PCR-RDB) to detect HPV.@*Methods@#HPV genotypes were detected in 316 paraffin specimens of confirmed or suspected perianal condyloma acuminata(CA) patients, including 58 cases for HPV DNA sequencing.@*Results@#The HPV infection rate in 316 patients was 81.6%. The first fifth genotypes of single HPV infection were HPV11, 6, 18, 16 and 33. HPV6+ 11, HPV6+ 11+ 16 and HPV6+ 18 were more common in mixed infection. The highest HPV infection rate was from the males aged below 35 years. Regarding the sequencing result as a gold standard, the sensitivity, specificity and accuracy of PCR-RDB in HPV were 92.31%, 89.47% and 91.38% respectively.@*Conclusions@#The proportion of HPV crissum infection in young male was quite high, the major pathogenic types were HPV 11, 6, 18, 16 and 33. The PCR-RDB method can meet the needs of clinical diagnosis of HPV.