Comparisons for kits HAVAb2.0 and HAV-IgG / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#Two kits for detecting anti-HAV antibody produced by Abbott Laboratories are evaluated for their performance in order to use in the anti-HAV antibodies detections.@*Methods@#Serially diluted standard reference serum were detected 4 times with HAVAb2.0 kit and HAV-IgG kit , then fit standard curves and calculated Interclass correlation coefficient (ICC). 120 serum that have different levels of anti-HAV antibodies were chosen to be detected by two kits and calculated sensitivity and specificity, receive operation characteristic (ROC) curve and area under curve (AUC) while the results of HAV-IgG were used as golden standard.@*Results@#R2 and ICC for HAV-IgG were 0.9977 and 0.999, respectively, higher than 0.9893 and 0.995(P>0.05) for HAVAb2.0. Sensitivity and specificity for HAVAb2.0 were 93.15% and 100% and AUC was 0.994 when kit HAV-IgG was chosen as golden standard.@*Conclusion@#There is little difference in performance between HAV-IgG and HAVAb2.0, both of them can be used in anti-HAV antibodies detection.