Research of multi-tube composite negative pressure suction device for the treatment of venous congestion flap / 中华整形外科杂志

ABSTRACT

Objective@#To compare the multi-tube composite negative pressure suction device with a single tube negative pressure suction device for the treatment of venous congestion flap.@*Methods@#15 New Zealand white rabbits were selected. Two lower abdomen skin flaps were larvested as venous congestion flap models in each rabbit. The 30 flaps were randomly divided into three groups, as group A(multi-tube composite negative pressure suction device, 10 flaps), group B(the single tube negative pressure suction device, 10 flaps) and group C(no pressure suction device, 10 flaps). The 10 flaps in each group were again randomly divided into six flaps for histological study and four flaps for observation. The six flaps were harvested at 4, 8, 24 hours and 3, 7 d after operation for detection of S-phase ratio(S-phase fraction, SPF) and cell apoptosis by flow cytometry. The 4 flaps were observed for flaps color, swelling, finger pressure reaction. The survival rate was measured 7 days after operation.@*Results@#The difference between group A and B or between B and C was significant in surface temperature (group A> B>C, P<0.05), flap survival area(group A> B>C, P<0.05), flap SPF(group A> B>C, P<0.05) and flap early apoptosis(group A< B<C, P<0.05).@*Conclusions@#Compostie negative pressure suction device can treat venous congestion flap, especially multi-tube device. It can promote flap tissue cell proliferation, reduce the apoptosis and increase the flap survival area.