Molecular evolutionary characteristics analysis of enterovirus 71 in Xinjiang Production and Construction Corps from 2011 to 2016 / 中华实验和临床病毒学杂志

ABSTRACT

Objective@#To analyze the phylogenetic characteristics of enterovirus 71 (EV71) in Xinjiang Production and Construction Corps from 2011 to 2016, providing pathogenic information for prevention and control of hand-foot-mouth disease (HFMD).@*Methods@#The EV71 positive strains were conducted for reverse transcription polymerase chain reactions (RT-PCR) amplification on entire VP1 coding region and sequencing was then performed and finally phylogcnetic tree was constructed among these strains and representative strains from GenBank using MEGA6.06.@*Results@#The homology of nucleotide and amino acid of the 37 EV71 strains were 87.3%-100.0% and 93.1%-100.0%, and belonged to EV71 C4a subgenotype, of 7 relatively independent small branches. Of the epidemic strains, compared with the representative strains, mutation occurred on 28 amino acid sites, the variation (Serine to Threonine, Alanine to Serine) happened on the 283 and 293 amino acid site in 9 strains.@*Conclusions@#The 37 EV71 strains in Xinjiang Production and Construction Corps from 2011 to 2016 belonged to EV71 C4a subgenotype, mutation occurred on 28 amino acid sites, and there is a common variation on the 283 and 293 amino acid site in 9 strains.