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Isolation and identification of Getah virus from Culicoides in Yunnan / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 405-408, 2017.
Article in Chinese | WPRIM | ID: wpr-808648
ABSTRACT
Objective@#To discuss the taxonomy and molecular characteristics of one virus strain (SZC30) isolated from Culicoides in Yunnan.@*Methods@#Culicoides were collected with light trap method in the Wulong Village of Shizong County of Yunnan Province in July, 2013. BHK-21 and C6/36 cells were used for virus isolation. The positive isolates were inoculated into brain of one-day suckling mice. Alphavirus and Getah virus specific primers were used to amplify the genome of the virus isolation by RT-PCR. The products of RT-PCR were sequenced. Clustal X1.83, DNAStar, Mega5.1 were used for bioinformatics analysis.@*Results@#Totally 3 500 culicoides were collected and divided into 41 batches for virus isolation. One isolate (SZC30) produced cytopathic effect (CPE) on BHK-21 and C6/36 cells; the result of RT-PCR with Alphavirus and Getah virus NS1 specific primer were positive; the sequence analysis of NS1 gene suggested that SZC30 and two Getah virus strains (YN0540, SC1210) from China were in the same evolutionary branching, the nucleotide homology were 97%-100%, and the amino acid was 97%-100%.@*Conclusions@#SZC30 isolated in Yunnan province was identified as Getah virus.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Prognostic study Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2017 Type: Article