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Oxidative Stress Modulates the Expression Pattern of Peroxiredoxin-6 in Peripheral Blood Mononuclear Cells of Asthmatic Patients and Bronchial Epithelial Cells
Allergy, Asthma & Immunology Research ; : 523-536, 2020.
Article in English | WPRIM | ID: wpr-811061
ABSTRACT

PURPOSE:

Reduction-oxidation reaction homeostasis is vital for regulating inflammatory conditions and its dysregulation may affect the pathogenesis of chronic airway inflammatory diseases such as asthma. Peroxiredoxin-6, an important intracellular anti-oxidant molecule, is reported to be highly expressed in the airways and lungs. The aim of this study was to analyze the expression pattern of peroxiredoxin-6 in the peripheral blood mononuclear cells (PBMCs) of asthmatic patients and in bronchial epithelial cells (BECs).

METHODS:

The expression levels and modifications of peroxiredoxin-6 were evaluated in PBMCs from 22 asthmatic patients. Phosphorylated and acetylated peroxiredoxin-6 in hydrogen peroxide-treated human BECs was detected using immunoprecipitation analysis. The expression level of peroxiredoxin-6 was also investigated in BECs treated with hydrogen peroxide. Cycloheximide and proteasome inhibitors were used to determine whether peroxiredoxin-6 is degraded by proteasomes.

RESULTS:

Peroxiredoxin-6 expression was significantly reduced in the PBMCs of asthmatic patients compared to control subjects. Distinct modification patterns for peroxiredoxin-6 were observed in the PBMCs of asthmatic patients using 2-dimensional-electrophoresis. The levels of phosphorylated serine and acetylated lysine in peroxiredoxin-6 were significantly increased in the BECs following hydrogen peroxide treatment. The level of peroxiredoxin-6 expression was reduced in hydrogen peroxide-stimulated BECs, presumably due to proteasomes.

CONCLUSIONS:

The expression of peroxiredoxin-6, which is down-regulated in the immune cells of asthmatic patients and BECs, can be modified by oxidative stress. This phenomenon may have an effect on asthmatic airway inflammation.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Asthma / Serine / Protein Processing, Post-Translational / Oxidative Stress / Cycloheximide / Immunoprecipitation / Epithelial Cells / Proteasome Inhibitors / Homeostasis / Hydrogen Limits: Humans Language: English Journal: Allergy, Asthma & Immunology Research Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Asthma / Serine / Protein Processing, Post-Translational / Oxidative Stress / Cycloheximide / Immunoprecipitation / Epithelial Cells / Proteasome Inhibitors / Homeostasis / Hydrogen Limits: Humans Language: English Journal: Allergy, Asthma & Immunology Research Year: 2020 Type: Article