A Novel Pancreatic Imaging Window for Stabilized Longitudinal In Vivo Observation of Pancreatic Islets in Murine Model
Diabetes & Metabolism Journal
;
: 193-198, 2020.
Article
in English
| WPRIM
| ID: wpr-811136
ABSTRACT
Longitudinal imaging of murine pancreas is technically challenging due to the mechanical softness of the tissue influenced by peristalsis. Here, we report a novel pancreatic imaging window for long-term stabilized cellular-level observation of the islets in the pancreas in vivo. By spatially separating the pancreas from the bowel movement and physiologic respiration with a metal plate integrated in the imaging window, we successfully tracked the pancreatic islets up to three weeks and visualized the dumbbell-shape transformation from the single islet. This window can be a useful tool for long-term cellular-level visualization of the microstructure in the pancreas.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreas
/
Peristalsis
/
Respiration
/
Islets of Langerhans
/
Intravital Microscopy
Limits:
Animals
Language:
English
Journal:
Diabetes & Metabolism Journal
Year:
2020
Type:
Article
Similar
MEDLINE
...
LILACS
LIS