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MiR-484 Protects Rat Myocardial Cells from Ischemia-Reperfusion Injury by Inhibiting Caspase-3 and Caspase-9 during Apoptosis
Korean Circulation Journal ; : 250-263, 2020.
Article in English | WPRIM | ID: wpr-811353
ABSTRACT
BACKGROUND AND

OBJECTIVES:

To reveal the detail mechanism of miR-484 on myocardial ischemia-reperfusion (MI/R) injury.

METHODS:

Rats model of MI/R injury was established based on control (Con; sham operate) group, ischemia-reperfusion (I/R) group, miR-484 treatment (miR) group, and I/R-negative control (IR-C) group, followed by pathological and interleukin (IL)-6, tumor necrosis factor (TNF)-α, and IL-1β expression evaluation. Then the myocardial apoptosis, as well as the expression of miR-484, caspase-3, and caspase-9 in myocardium were examined. Finally, the regulatory relation between miR-484 and SMAD family member 7 (SMAD7) was predicated, followed by verification analysis.

RESULTS:

Compared with Con group, the expression of miR-484 in I/R and IR-C group was decreased. Compared with I/R and IR-C group, the expression of miR-484 was increased in miR group. Compared with Con group, the expression levels of IL-6, TNF-α, and IL-1β in cardiac myocytes of I/R group and IR-C group were increased. Compared with Con group, the apoptotic index, membrane potential of I/R, and the expression of caspase-3/9 were increased in IR-C group. Compared with the I/R and IR-C groups, the apoptotic index of myocardial cells in the ischemic region was decreased, the membrane potential was increased, and the expression of caspase-3/9 was decreased significantly in the miR group. SMAD7 was the target gene of miR-484.

CONCLUSIONS:

MiR-484 protected myocardial cells from I/R injury by suppressing caspase-3 and caspase-9 expression during cardiomyocyte apoptosis. MiR-484 reduced the expression of IL-6, TNF-α, and IL-1β in MI/R. MiR-484 might alleviate the decreasing of mitochondrial membrane potential in MI/R cells.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Reperfusion Injury / Interleukins / Interleukin-6 / Tumor Necrosis Factor-alpha / Apoptosis / Myocytes, Cardiac / Caspase 3 / Caspase 9 / Membrane Potential, Mitochondrial / Membrane Potentials Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Korean Circulation Journal Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Reperfusion Injury / Interleukins / Interleukin-6 / Tumor Necrosis Factor-alpha / Apoptosis / Myocytes, Cardiac / Caspase 3 / Caspase 9 / Membrane Potential, Mitochondrial / Membrane Potentials Type of study: Prognostic study Limits: Animals / Humans Language: English Journal: Korean Circulation Journal Year: 2020 Type: Article