Inhibitions and mechanisms of amygdalin in non-small cell lung cancer NCI-H1299 cell line invasion in vitro / 中国药科大学学报
Journal of China Pharmaceutical University
;
(6): 479-482, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-811849
ABSTRACT
@#The aim of this study was to investigate the effects and mechanisms of a nature product, amygdalin, in non-small cell lung cancer NCI-H1299 cell line invasion and migration in vitro. NCI-H1299(H1299)cells were treated with amygdalin. MTS assay was employed to determine cell proliferation, transwell chamber and wound-healing assay were employed to determine invasion and migration in vitro, western blotting and RT-PCR assay were employed to determine the expression of integrin β1, integrin β4, integrin-linked kinase(ILK), focal adhesion kinase(FAK), p-FAK and β-catenin, and the phosphorylation of Akt and RICTOR. Results showed that in vitro proliferation of H1299 cells were inhibited significantly after treated with 0. 5 and 1 mg/mL amygdalin for 48 h. The invasion and migration potential was decreased. The protein and mRNA expression of MMP-2/9, integrin β1/4, ILK, FAK, β-catenin, the activity of MMP-2/9, the phosphorylation of FAK, Akt and RICTOR were all decreased significantly(all of P< 0. 05).
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of China Pharmaceutical University
Year:
2016
Type:
Article
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