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Molecular diversity analysis of Tetradium ruticarpum (WuZhuYu) in China based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 1-9, 2018.
Article in English | WPRIM | ID: wpr-812034
ABSTRACT
"Wu zhu yu", which is obtained from the dried unripe fruits of Tetradium ruticarpum (A. Jussieu) T. G. Hartley, has been used as a traditional Chinese medicine for treatment of headaches, abdominal colic, and hypertension for thousands of years. The present study was designed to assess the molecular genetic diversity among 25 collected accessions of T. ruticarpum (Wu zhu yu in Chinese) from different areas of China, based on inter-primer binding site (iPBS) markers and inter-simple sequence repeat (ISSR) markers. Thirteen ISSR primers generated 151 amplification bands, of which 130 were polymorphic. Out of 165 bands that were amplified using 10 iPBS primers, 152 were polymorphic. The iPBS markers displayed a higher proportion of polymorphic loci (PPL = 92.5%) than the ISSR markers (PPL = 84.9%). The results showed that T. ruticarpum possessed high loci polymorphism and genetic differentiation occurred in this plant. The combined data of iPBS and ISSR markers scored on 25 accessions produced five clusters that approximately matched the geographic distribution of the species. The results indicated that both iPBS and ISSR markers were reliable and effective tools for analyzing the genetic diversity in T. ruticarpum.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Polymorphism, Genetic / Genetic Variation / Binding Sites / Base Sequence / Genetic Markers / DNA Fingerprinting / Classification / DNA Primers / DNA, Plant Language: English Journal: Chinese Journal of Natural Medicines (English Ed.) Year: 2018 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Phylogeny / Polymorphism, Genetic / Genetic Variation / Binding Sites / Base Sequence / Genetic Markers / DNA Fingerprinting / Classification / DNA Primers / DNA, Plant Language: English Journal: Chinese Journal of Natural Medicines (English Ed.) Year: 2018 Type: Article