Development of a quantitative ELISA kit for human secreted CD306/LAIR-2 and its application / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 847-852, 2014.
Article
in English
| WPRIM
| ID: wpr-812192
ABSTRACT
AIM@#A quantitative ELISA kit for the detection of human secreted CD306/LAIR-2 was developed using monoclonal and polyclonal antibodies which were raised against a highly purified recombinant human secreted CD306/LAIR-2.@*METHODS@#Anti-human secreted CD306/LAIR-2 monoclonal and polyclonal antibodies were raised by immunizing mouse or rabbit with recombinant human secreted CD306/LAIR-2. The monoclonal antibody was purified by protein G affinity, whereas the polyclonal antibody was purified by protein A affinity. The best match pair of antibodies were found and used to develop a double antibody sandwich ELISA kit for the detection of human secreted CD306/LAIR-2 in human samples.@*RESULTS@#A human secreted CD306/LAIR-2 ELISA kit was formulated with highly purified recombinant human secreted CD306/LAIR-2, highly specific monoclonal and polyclonal antibodies. This kit realized the quantitative measurement of recombinant human CD306/LAIR-2 and natural CD306/LAIR-2 in human serum samples.@*CONCLUSIONS@#The developed human secreted CD306/LAIR-2 ELISA kit is a reliable quantitation immunoassay kit.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Recombinant Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Receptors, Immunologic
/
Allergy and Immunology
/
Methods
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2014
Type:
Article
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