Tectorigenin inhibits the inflammation of LPS-induced acute lung injury in mice / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 841-846, 2014.
Article
in English
| WPRIM
| ID: wpr-812193
ABSTRACT
AIM@#In a previous study, the anti-inflammatory effects of tectorigenin were disclosed. In this study, the anti-inflammatory effects of tectorigenin on acute lung injury using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model were investigated@*METHOD@#The cell-count in the bronchoalveolar lavage fluid (BALF) was measured. The animal lung edema degree was evaluated by the wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) activity and myeloperoxidase (MPO) activity was assayed using SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α), IL-1β, and IL-6 were assayed using an enzyme-linked immunosorbent assay method. Pathological changes of lung tissues were observed through HE staining. The inflammatory signal pathway related protein nuclear factor NF-κB p65 mRNA expression was measured by real-time PCR, and the protein level of NF-κB p65 was measured using Western blotting analysis.@*RESULTS@#The data showed that treatment with the tectorigenin markedly attenuated the inflammatory cell numbers in the BALF, decreased nuclear factor NF-κB p65 mRNA level and protein level in the lungs, and improved SOD activity and inhibited MPO activity. Histological studies showed that tectorigenin substantially inhibited LPS-induced neutrophils in lung tissue compared with the model group.@*CONCLUSION@#The results indicated that tectorigenin had a protective effect on LPS-induced ALI in mice.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pulmonary Edema
/
Superoxide Dismutase
/
Bronchoalveolar Lavage Fluid
/
Cell Count
/
Lipopolysaccharides
/
Peroxidase
/
Cell Biology
/
Therapeutic Uses
/
Drug Therapy
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2014
Type:
Article
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