Simultaneous quantitative determination of five alkaloids in Catharanthus roseus by HPLC-ESI-MS/MS / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 786-793, 2014.
Article
in English
| WPRIM
| ID: wpr-812199
ABSTRACT
AIM@#To establish a method to simultaneously determine the main five alkaloids of Catharanthus roseus for trace samples, a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) analysis method was developed.@*METHOD@#The five Catharanthus alkaloids, vinblastine, vincristine, vinleurosine, vindoline, and catharanthine were chromatographically separated on a C18 HPLC column. The mobile phase was methanol-15 nmol·L(-1) ammonium acetate containing 0.02% formic acid (65 35, V/V). The quantification of these alkaloids was based on the Multiple Reaction Monitoring (MRM) mode.@*RESULTS@#This method was validated, and the results achieved the aims of the study. The intra- and inter-day precision and accuracy of the five alkaloids were within 1.2%-11.5% (RSD%) and -10.9%-10.5% (RE%). The recovery rates of the five alkaloids of samples were from 79.9% to 91.5%. The five analytes were stable at room temperature for 2 h, at 4 °C for 12 h, and at -20 °C for two weeks. The developed method was applied successfully to determine the content of the five alkaloids in three plant parts of three batches of C. roseus with a minute amount collected from three regions of China.@*CONCLUSION@#The HPLC-ESI-MS/MS method can be used for the simultaneous determination of five important alkaloids in trace C. roseus samples.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Drugs, Chinese Herbal
/
Chemistry
/
China
/
Chromatography, High Pressure Liquid
/
Catharanthus
/
Alkaloids
/
Tandem Mass Spectrometry
/
Methods
Country/Region as subject:
Asia
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2014
Type:
Article
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