Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 572-576, 2013.
Article
in English
| WPRIM
| ID: wpr-812318
ABSTRACT
AIM@#To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.@*METHODS@#Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.@*RESULTS@#The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).@*CONCLUSION@#The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pollen
/
Blood
/
Drugs, Chinese Herbal
/
Pharmacokinetics
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Electrospray Ionization
/
Typhaceae
/
Flavonols
/
Tandem Mass Spectrometry
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2013
Type:
Article
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