Sangxingtang inhibits the inflammation of LPS-induced acute lung injury in mice by down-regulating the MAPK/NF-κB pathway / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
; (6): 889-895, 2015.
Article
in En
| WPRIM
| ID: wpr-812467
Responsible library:
WPRO
ABSTRACT
In the present study, we investigated anti-inflammatory effects of Sangxingtang (SXT) on acute lung injury using a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. The cell counting in the bronchoalveolar lavage fluid (BALF) was performed. The degree of lung edema was evaluated by measuring the wet/dry weight (W/D) ratio. The superoxidase dismutase (SOD) and myeloperoxidase (MPO) activities were assayed by SOD and MPO kits, respectively. The levels of inflammatory mediators, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), were assayed by the enzyme-linked immunosorbent assay methods. Pathological changes of lung tissues were observed by Hematoxylin and eosin (HE) staining. The inflammatory signaling pathway-related proteins nuclear factor mitogen activated protein kinases (P38MAPK), extracellular regulated protein kinases (Erk), c-Jun N-terminal kinase (Jnk) and nuclear transcription factor (NF-κB) p65 expressions were measured by Western blotting. Our results showed that the treatment with the SXT markedly attenuated the inflammatory cell numbers in the BALF, decreased the levels of P-P38MAPK, P-Erk, P-Jnk and P-NF-κB p65 and the total protein levels in lungs, improved the SOD activity and inhibited the MPO activity. Histological studies demonstrated that SXT substantially reduced the LPS-induced neutrophils in lung tissues, compared with the untreated LPS group. In conclusion, our results indicated that SXT had protective effects on LPS-induced ALI in mice.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Drugs, Chinese Herbal
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Signal Transduction
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Down-Regulation
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Lipopolysaccharides
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Tumor Necrosis Factor-alpha
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Mitogen-Activated Protein Kinases
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Drug Therapy
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Allergy and Immunology
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Acute Lung Injury
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Genetics
Type of study:
Prognostic_studies
Limits:
Animals
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Female
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Humans
Language:
En
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2015
Type:
Article