Six new cytotoxic and anti-inflammatory 11, 20-epoxy-ent-kaurane diterpenoids from Isodon wikstroemioides / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 383-389, 2015.
Article
in English
| WPRIM
| ID: wpr-812531
ABSTRACT
The present study was designed to determine the chemical constituents of EtOAc extracts of the aerial parts of Isodon wikstroemioides. Compounds 1-8 were isolated and purified by normal-phase silica gel and reversed-phase C18silica gel column chromatography and HPLC. Their structures were elucidated by extensive spectroscopic methods. Most of them were evaluated for their in vitro cytotoxicity against human cancer HL-60, SMMC-7721, A-549, MCF-7, and SW-480 cells and their inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. Among the eight 11, 20-epoxy-ent-kauranoids isolated, compounds 1-6 (isowikstroemins H-M) were new diterpenoids. Compounds 1, 3, and 7 exhibited significant cytotoxicity with IC50 values ranging from (0.84 ± 0.02) to (4.09 ± 0.34) μmol · L(-1), while compounds 4 and 5 showed selective cytotoxicity. In addition, compounds 1, 3, 4, and 7 exhibited inhibitory activity against nitric oxide (NO) production in LPS-activated RAW264.7 macrophages. These results provide a basis for future development of these compounds as anti-cancer and anti-inflammatory agents.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plant Extracts
/
Chemistry
/
Lipopolysaccharides
/
Inhibitory Concentration 50
/
Isodon
/
Plant Components, Aerial
/
Cell Line, Tumor
/
Diterpenes, Kaurane
/
Drug Therapy
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2015
Type:
Article
Similar
MEDLINE
...
LILACS
LIS