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NAMPT inhibition synergizes with NQO1-targeting agents in inducing apoptotic cell death in non-small cell lung cancer cells / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.) ; (6): 582-589, 2016.
Article in English | WPRIM | ID: wpr-812589
ABSTRACT
Nicotinamide phosphoribosyltransferase (NAMPT) catalyzes the first rate-limiting step in converting nicotinamide to NAD(+), essential for a number of enzymes and regulatory proteins involved in a variety of cellular processes, including deacetylation enzyme SIRT1 which modulates several tumor suppressors such as p53 and FOXO. Herein we report that NQO1 substrates Tanshione IIA (TSA) and β-lapachone (β-lap) induced a rapid depletion of NAD(+) pool but adaptively a significant upregulation of NAMPT. NAMPT inhibition by FK866 at a nontoxic dose significantly enhanced NQO1-targeting agent-induced apoptotic cell death. Compared with TSA or β-lap treatment alone, co-treatment with FK866 induced a more dramatic depletion of NAD(+), repression of SIRT1 activity, and thereby the increased accumulation of acetylated FOXO1 and the activation of apoptotic pathway. In conclusion, the results from the present study support that NAMPT inhibition can synergize with NQO1 activation to induce apoptotic cell death, thereby providing a new rationale for the development of combinative therapeutic drugs in combating non-small lung cancer.
Subject(s)

Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cytokines / Naphthoquinones / NAD(P)H Dehydrogenase (Quinone) / Apoptosis / Carcinoma, Non-Small-Cell Lung / Cell Line, Tumor / Abietanes / Drug Therapy / Enzyme Inhibitors Limits: Humans Language: English Journal: Chinese Journal of Natural Medicines (English Ed.) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Cytokines / Naphthoquinones / NAD(P)H Dehydrogenase (Quinone) / Apoptosis / Carcinoma, Non-Small-Cell Lung / Cell Line, Tumor / Abietanes / Drug Therapy / Enzyme Inhibitors Limits: Humans Language: English Journal: Chinese Journal of Natural Medicines (English Ed.) Year: 2016 Type: Article