In vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice / 中国天然药物
Chinese Journal of Natural Medicines (English Ed.)
;
(6): 472-476, 2013.
Article
in English
| WPRIM
| ID: wpr-812635
ABSTRACT
AIM@#To study the in vivo anti-inflammatory activity of Tabernaemontana divaricata leaf extract on male albino mice.@*METHODS@#Aqueous decoction and methanol leaf extracts were tested for their ability to reduce croton oil-induced edema in the mouse ear after topical application. The methanol leaf extract dose-dependently inhibited the croton oil-induced ear edema in mice (ID50 <500 μg·cm(-2)). A bioassay-guided liquid-liquid fractionation of this methanol extract gave four active fractions water insoluble (F1), hexane (F2), ethyl acetate (F3) and water (F4).@*RESULTS@#The hexane fraction showed a very high activity (42.1% inhibition at 0.7 μg·cm(-2)) as compared to the control. The other fractions were less active (F1 56.1% at 506.2 μg·cm(-2); F3 57.3% at 289.3 μg·cm(-2); and F4 31.9% for 203.8 μg·cm(-2)) while indomethacin gave 48.8% of inhibition at 90 μg·cm(-2). The activity of F1 and F3 may be at least in part explained by the presence of anti-inflammatory flavonoids, while the activity was not correlated to the tannin contents. No compounds were detected in the most active F2 fraction.@*CONCLUSIONS@#The results give a rational support to the traditional use of T. divaricata in tropical India as anti-inflammatory agent.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plant Extracts
/
Chemistry
/
Plant Leaves
/
Tabernaemontana
/
Drug Therapy
/
Edema
/
Anti-Inflammatory Agents
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Chinese Journal of Natural Medicines (English Ed.)
Year:
2013
Type:
Article
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