H₂O₂ alters metabolism in TM4 Sertoli cells in the mouse / 中华男科学杂志
National Journal of Andrology
;
(12): 497-502, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-812735
ABSTRACT
Objective@#To explore the mechanisms of oxidative stress-induced damage to TM4 Sertoli cells in the mouse using metabolomics techniques based on gas chromatography-mass spectrometry (GC-MS).@*METHODS@#We established the model of oxidative stress-induced damage to mouse TM4 Sertoli cells by treatment with H₂O₂. Then, we detected the survival rate and apoptosis rate of the TM4 cells by MTT and flow cytometry respectively, measured the concentration of ROS in the TM4 cells with the DCFH-DA fluorescent probe, and determined the levels of endogenous metabolites in the TM4 cells by GC-MS after H₂O₂ intervention.@*RESULTS@#After 2 hours of treatment with H₂O₂ at 600 μmol/L, the survival rate of the TM4 cells was reduced to about 50%, and the total apoptosis rates in the low- (100 μmol/L), medium- (300 μmol/L), and high-dose (600 μmol/L) groups were (19.45 ± 0.53), (20.12 ± 0.58), and (37.13 ± 0.35)%, respectively, increased in a dose-dependent manner as compared with (10.28 ± 0.35)% in the blank control (P 1, P <0.05).@*CONCLUSIONS@#Oxidative stress-induced damage and apoptosis of TM4 Sertoli cells are closely associated with the metabolism of amino acid, glucose, and energy in the cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sertoli Cells
/
Time Factors
/
Cell Survival
/
Reactive Oxygen Species
/
Apoptosis
/
Oxidative Stress
/
Dose-Response Relationship, Drug
/
Energy Metabolism
/
Metabolomics
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2017
Type:
Article
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