Yam polysaccharide improves sperm viability and protects sperm DNA integrity in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 1020-1024, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-812839
ABSTRACT
Objective@#To clarify the roles of yam polysaccharide (YPS) in improving sperm viability and protecting sperm DNA integrity in vitro and provide a new approach to the treatment of oligoasthenozoospermia.@*METHODS@#We collected samples by masturbation from 36 normal fertile males aged 27-39 years. Each sample was divided into six groups blank control or treated with normal saline, vitamin C solution, and YPS solution at low (0.25 mg/ml), medium (1.0 mg/ml) or high concentration (5.0 mg/ml). Using eosin-Y staining, sperm hypotonic swelling (HOS) and sperm chromatin diffusion (SCD) test, we observed the effects of different concentrations of YPS on sperm viability, membrane integrity and nuclear DNA.@*RESULTS@#After 24 and 48 hours of treatment, sperm viability was markedly reduced in the vitamin C ([28.5 ± 3.1] and [6.5 ± 1.2]%), low-YPS ([31.3 ± 3.5] and [6.5 ± 2.2]%), medium-YPS ([37.1 ± 3.5] and [9.5 ± 2.8]%) and high-YPS groups ([38.3 ± 3.3] and [9.0 ± 3.2]%) as compared with the blank control ([17.3 ± 2.1] and [3.2 ± 1.3]%) (P 0.05).@*CONCLUSIONS@#Yam polysaccharide can improve sperm viability and protect sperm DNA integrity in vitro.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Polysaccharides
/
Ascorbic Acid
/
Sperm Motility
/
Spermatozoa
/
Vitamins
/
DNA
/
Chemistry
/
Dioscorea
Limits:
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2017
Type:
Article
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